Viral tracing of innervation
نویسندگان
چکیده
منابع مشابه
Neuro-tracing approach to study kidney innervation: a technical note
Neuro-tracing approach is a great option to study innervation of the visceral organs including the kidneys. Important factors contributing to the success of this technique include the choice of a neuro-tracer, and delivery methods to result in successful labeling of peripheral sensory and motor ganglia. The neuro-tracer is usually applied directly to the kidney accessed via a surgical opening o...
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Classical methods for studying neuronal circuits are fairly low throughput. Transsynaptic viruses, particularly the pseudorabies (PRV) and rabies virus (RABV), and more recently vesicular stomatitis virus (VSV), for studying circuitry, is becoming increasingly popular. These higher throughput methods use viruses that transmit between neurons in either the anterograde or retrograde direction. Re...
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Previous studies demonstrated that strain H129 of herpes simplex virus-1 undergoes anterograde transneuronal transport in mice and primates after peripheral or central injection. In this study, H129 was used in rats to identify CNS regions that receive relayed viscero-sensory inputs from the stomach wall. We also examined whether transneuronal viral transport in this model is exclusively antero...
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Previously reported multisynaptic tracing of the mouse eyeblink circuit [1] revealed characteristic spatiotemporal patterns in cerebellar labelling that suggested well-defined corticonuclear pathways. This project further explores the connectivity of these circuits in the cerebellum and in downstream motor structures. Cerebellar cortical projections to the deep cerebellar nuclei were traced wit...
متن کاملInnervation of the superior tarsal (Müller's) muscle in the cynomolgus monkey: a retrograde tracing study.
PURPOSE The retrograde transport of wheat germ agglutinin-horseradish peroxidase (WGA/HRP) was used to study the localization of the neurons that innervate the superior tarsal muscle in the cynomolgus monkey. METHODS A 5-10% WGA-HRP solution was applied to the medial part of the superior tarsal muscle. Seventy-two hours later, the animals were killed and perfused with fixative. RESULTS The ...
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ژورنال
عنوان ژورنال: Science
سال: 1994
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.8016646